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KMID : 0360220130540060945
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Journal of the Korean Ophthalmological Society
2013 Volume.54 No. 6 p.945 ~ p.953
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Expression Profiles of F4/80 and Nestin in Ocular Immune Cells Following Pharmaceutically Induced Retinal Degeneration in Adult Mice
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Ahn Sang-Il
Ohn Young-Hoon
Park Tae-Kwann
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Abstract
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Purpose: To evaluate the expression patterns of F4/80 and nestin in the ciliary body and the optic nerve following N-methyl- N-nitrosourea (NMU)-induced retinal degeneration in adult mice. Methods: After intraperitoneal injection of MNU (60 mg/kg) in adult mice, the eyes were enucleated at 2, 4, 7 and 30 days. Hematoxylin and eosin (H&E) stain, terminal deoxyribonucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) stain and immunohistochemical stains of F/80 and nestin were performed. Results: After MNU treatment, the photoreceptors were destroyed by cell apoptosis. According to immunohistochemistry, F4/80 and nestin were not co-expressed in the control group, but F4/80 was expressed within the ciliary body and optic nerve in the MNU-treated group; the expression of nestin also increased. In the outer nuclear layer, F4/80 and nestin co-expressing cells were observed. Conclusions: In response to retinal damage, the F4/80 and nestin co-expressing cells migrated to the retina from the ciliary body and optic nerve and were activated.
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KEYWORD
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F4/80, MNU, Nestin, Retinal degeneration
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